Taq DNA Ligase from New England Biolabs

Product Specs
ItemTaq DNA Ligase
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0208L
Quantity10000 units
Concentration40,000 units/ml
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeEnzymes
Temperature-20 C

Specifications/Features

Unit Definition:
(Cohesive End Unit)
One unit is defined as the amount of enzyme required to give 50% ligation of the 12-base pair cohesive ends of 1 μg of BstEII-digested λ DNA in a total reaction volume of 50 μl in 15 minutes at 45°C.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Engineering E. coli strains using antibiotic-resistance-gene-free plasmidsCell Rep MethodsDecember 18, 2023Matthew B. Amrofell, Sunaina Rengarajan, Steven T. Vo, Erick S. Ramirez Tovar, Larissa LoBello, Gautam Dantas, Tae Seok Moon,New England Biolabs,M0202 T5 exonuclease,New England Biolabs,M0663 Taq DNA Ligase,New England Biolabs,M0208 Thiamine hydrochloride,Sigma,T1270 Thymidine (Thy),Sigma,T1895 Tris base,Sigma,77-86-1 Tryptone

(2) H4K20me3 is important for Ash1-mediated H3K36me3 and transcriptional silencing in facultative heterochromatin in a fungal pathogenPLOS GeneticsSeptember 25, 2023Mareike Möller, John B. Ridenour, Devin F. Wright, Faith A. Martin, Michael FreitagThermoFisher #F530L], 3.125 μL ET SSB [500 μg/ml NEB #M2401S], 50 μL Taq DNA-Ligase [40 U/μL stock NEB #M0208L]). Primers, plasmids, and strains used in this study are listed in S4 Table. Transformation of Zymoseptoria

(3) Development of Zika Virus E Variants for Pseudotyping Retroviral Vectors Targeting Glioblastoma CellsInternational journal of molecular sciencesSeptember 23, 2023Grunwald V, Ngo HD, Formanski JP, Jonas JS, Pöhlking C, Schwalbe B, Schreiber M.Main, Germany, #M0363), 4 µL of Phusion DNA Polymerase (NEB, #M0530), 32 µL of Taq DNA Ligase (NEB, #M0208), and 140 µL of H2O. The Gibson 5× ISO buffer was made from 3 mL of Tris pH 7.5 (1 M), 150 µL of MgCl2

(4) The ER membrane protein complex governs lysosomal turnover of a mitochondrial tail-anchored protein, BNIP3, to restrict mitophagybioRxiv : the preprint server for biologyMarch 24, 2023Delgado JM, Wallace Shepard L, Lamson SW, Liu SL, Shoemaker CJ.orthovanadate (450243–10G, Sigma), sodium pyrophosphate decahydrate (221368–100G, Sigma), Taq DNA ligase (M0208L, NEB), TERGITOL Type NP-40 (NP40S-100ML, Sigma), Tris base (T1378–5KG, Sigma), TritonX-100 (T9284–500ML,

(5) Putative Signals of Generalist Plant Species Adaptation to Local Pollinator Communities and Abiotic FactorsMolecular Biology and EvolutionMarch 4, 2023Léa Frachon, Luca Arrigo, Quint Rusman, Lucy Poveda, Weihong Qi, Giovanni Scopece, Florian P Schiestlno. M0267S). After labeling, nicks were ligated with Taq DNA ligase (New England BioLabs, cat. no. M0208S) in the presence of deoxynucleotide triphosphates (dNTPs). The backbone of fluorescently labeled DNA

(6) In situ sequence-specific visualization of single methylated cytosine on tissue sections using ICON probe and rolling-circle amplificationHistochemistry and Cell BiologyMarch 1, 2023Sohei Kitazawa, Ryuma Haraguchi, Yuki Takaoka, Riko Kitazawa(padlock probe (1 pmol/µl) 5 µl, 10 × Taq-DNA buffer 5 µl, Taq DNA ligase (New England Biolabs, cat# M0208S) 2.5 µl, sterilized water 37.5 µl). The reaction solution was then layered on the section, treated at

(7) ESPRESSO: Robust discovery and quantification of transcript isoforms from error-prone long-read RNA-seq dataScience AdvancesJanuary 20, 2023Yuan Gao, Feng Wang, Robert Wang, Eric Kutschera, Yang Xu, Stephan Xie, Yuanyuan Wang, Kathryn E. Kadash-Edmondson, Lan Lin, Yi XingSecond-strand synthesis of LRCA products was performed using Phusion DNA polymerase and Taq DNA ligase (NEB, #M0208) by the gap-filling and ligation reaction at 50°C for 60 min. The dsDNA sequences were then sheared

(8) Structural basis for the assembly of the type V CRISPR-associated transposon complexCellDecember 22, 2022Michael Schmitz, Irma Querques, Seraina Oberli, Christelle Chanez, Martin JinekPhusion® High-Fidelity DNA Polymerase,New England Biolabs,Cat#M0530L Taq DNA Ligase,New England Biolabs,Cat#M0208L T4 DNA Ligase,New England Biolabs,Cat#M0202S 10x T4 DNA Ligase Reaction Buffer,New England Biolabs,Cat#B0202S BamHI,New England Biolabs

(9) Engineering a two-gene system to operate as a highly sensitive biosensor or a sharp switch upon induction with β-estradiolScientific ReportsDecember 16, 2022Tian Zhou, Zhiying Liang, Mario Andrea Marchisio(NEB-M0363), Phusion High-Fidelity DNA polymerase (Thermo Scientific-F530L), and Taq DNA ligase (NEB-M0208L)). The overall 20 µl solution was kept in a Thermal Cycler for 1 h at 50 °C. Escherichia coli competent

(10) Production and purification of recombinant monoclonal antibodies from human cells based on a primary sequenceSTAR ProtocolsDecember 16, 2022Keith F. DeLuca, Jeanne E. Mick, Jennifer G. DeLucaT5 Exonuclease,NEB,Cat# M0663S Phusion High-Fidelity DNA Polymerase,NEB,Cat# M0530S Taq DNA Ligase,NEB,Cat# M0208L NEB Gibson assembly master mix (alternative to making in-house isothermal assembly enzyme mix),NEB,Cat#

(11) Facile Assembly of Combinatorial Mutagenesis Libraries Using Nicking MutagenesisMethods in Molecular BiologyNovember 29, 2022Monica B. Kirby, Timothy A. WhiteheadPhusion High-Fidelity DNA Polymerase (New England Biolabs Cat # M0530S/L). 40 U/μL Taq DNA ligase (New England Biolabs Cat # M0208S/L). 20 U/μL DpnI (New England Biolabs Cat # R0176S/L). Shrimp Alkaline Phosphatase rSAP (New England Biolabs

(12) Morphology and gene expression profiling provide complementary information for mapping cell stateCell SystemsNovember 1, 2022Gregory P. Way, Ted Natoli, Adeniyi Adeboye, Lev Litichevskiy, Andrew Yang, Xiaodong Lu, Juan C. Caicedo, Beth A. Cimini, Kyle Karhohs, David J. Logan, et al.Kit,QIAGEN,Cat# 203209 M-MLV Reverse Transcriptase Kit,Promega,Cat# M1705 Taq Ligase Kit,NEB,Cat# M0208L MagPlex Microspheres,Luminex,N/A Cell Titer Glo,Promega,Cat#G7573 Deposited Data,, L1000 dataset

(13) In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assemblySTAR ProtocolsSeptember 16, 2022Andrew Wolff, Cynthia Wagner, Julia Wolf, Daniel LoboScientific,AC327190010 Magnesium chloride,Fisher Scientific,BP9741-10X5 Tris,VWR,97061-794 Taq DNA Ligase,New England Biolabs,M0208L T5 Exonuclease,New England Biolabs,M0663S Phusion High-Fidelity DNA Polymerase,New England Biolabs,M0530S Tryptone,Fisher

(14) The N Terminus of Adhesion G Protein–Coupled Receptor GPR126/ADGRG6 as Allosteric Force IntegratorFrontiers in Cell and Developmental BiologyJune 23, 2022Jakob Mitgau, Julius Franke, Camilla Schinner, Gabriele Stephan, Sandra Berndt, Dimitris G. Placantonakis, Hermann Kalwa, Volker Spindler, Caroline Wilde, Ines Liebscheraccording to the protocol. All enzymes were acquired from NEB: T5 exonuclease (M0363), Taq DNA Ligase (M0208), and Phusion DNA Polymerase (M0530). The WT receptor DNA for the assembly was obtained via PCR using

(15) Cohesin-dependence of neuronal gene expression relates to chromatin loop lengtheLifeApril 26, 2022Lesly Calderon, Felix D Weiss, Jonathan A Beagan, Marta S Oliveira, Radina Georgieva, Yi-Fang Wang, Thomas S Carroll, Gopuraja Dharmalingam, Wanfeng Gong, Kyoko Tossell, et al.then annealed in adjacent positions were ligated through the addition of 10 U (20 μL) Taq ligase (NEB M0208L) and incubation at 55 °C for 1 hr then 75 °C for 10 min. Successfully ligated primer-primer pairs were

(16) RIF1 acts in DNA repair through phosphopeptide recognition of 53BP1Molecular cellApril 7, 2022Setiaputra D, Escribano-Díaz C, Reinert JK, Sadana P, Zong D, Callen E, Sifri C, Seebacher J, Nussenzweig A, Thomä NH, et al.M0363S Phusion DNA polymerase,New England Biolabs,Cat#: M0530S Taq DNA ligase,New England Biolabs,Cat#: M0208S NAD,Sigma-Aldrich,Cat#: 10127965001 PEG8000,Sigma-Aldrich,Cat#: 81268 Experimental models: Cell lines,, Human:

(17) Engineering ligand-specific biosensors for aromatic amino acids and neurochemicalsCell SystemsMarch 1, 2022Austin G. Rottinghaus, Chenggang Xi, Matthew B. Amrofell, Hyojeong Yi, Tae Seok Moon,New England BioLabs,M0202 T5 exonuclease,New England BioLabs,M0663 Taq DNA Ligase,New England BioLabs,M0208 L-Threonine,Sigma,T8441 Tris base,Sigma,77-86-1 Tryptamine,Sigma,193747 L-Tryptophan,Sigma,T0254 Tyramine

(18) Bacteroides thetaiotaomicron uses a widespread extracellular DNase to promote bile-dependent biofilm formationProceedings of the National Academy of SciencesFebruary 15, 2022Nathalie Béchon, Jovana Mihajlovic, Anne-Aurélie Lopes, Sol Vendrell-Fernández, Julien Deschamps, Romain Briandet, Odile Sismeiro, Isabelle Martin-Verstraete, Bruno Dupuy, Jean-Marc Ghigo6.25 µL 2,000 U/mL Phusion HF polymerase (NEB No. M0530S), 50 µL 40,000 U/mL Taq DNA ligase (NEB No. M0208S), 87 µL dH2O for 24 reactions) and incubated at 50 °C for 35 min. The resulting mix was transformed

(19) Cantaloupe melon genome reveals 3D chromatin features and structural relationship with the ancestral cucurbitaceae karyotypeiScienceJanuary 21, 2022Clement Pichot, Anis Djari, Joseph Tran, Marion Verdenaud, William Marande, Cecile Huneau, Veronique Gautier, David Latrasse, Sandrine Arribat, Vivien Sommard, et al.Genomics,Cat#20168 Taq polymerase,New England Biolabs,Cat#M0267S Taq DNA Ligase,New England Biolabs,Cat#M0208S 10X ThermoPol Rxn Buffer,New England Biolabs,Cat#B9004S DNA Stain YOYO,Bionano Genomics,Cat#80005 Polylysine

(20) A composite DNA element that functions as a maintainer required for epigenetic inheritance of heterochromatinMolecular CellOctober 7, 2021Xiaoyi Wang, Joao A. Paulo, Xue Li, Haining Zhou, Juntao Yu, Steven P. Gygi, Danesh MoazedT5 Exonuclease,NEB,Cat #M0663 Phusion High-Fidelity DNA Polymerase,NEB,Cat #M0530S Taq DNA Ligase,NEB,Cat #M0208S DNA Size Selector I,Thermo Fisher Scientific,Cat# NC1148310 G418 sulfate,Thermo Fisher Scientific,Cat#

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Taq DNA Ligase

Taq DNA Ligase from New England Biolabs

Description

Specifications/Features

New England Biolabs

Citations (65)

Reviews

Taq DNA Ligase from New England Biolabs

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